Determination of ursolic acid in herba of Verbena officinalis by HPLC.
- Author:
Cheng-hong LIU
1
;
Yun LIU
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Plant Components, Aerial; chemistry; Plants, Medicinal; chemistry; Quality Control; Triterpenes; analysis; Verbena; chemistry
- From: China Journal of Chinese Materia Medica 2002;27(12):916-918
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a HPLC method for determination of ursolic acid in dried aerial part of Verbena officinalis.
METHODThe column used was a Kromasil C18 (4.6 mm x 250 mm) packed with a 5 microns stationary phase. The mobile phase consisted of methanol-sodium phosphate buffer [monobasic sodium phosphate (MW = 119.98) 1.7997 g and phosphoric acid (85%) 1.02 mL, combined and brought the total volume of 1,000 mL with water] (89:11); the mobile phase was maintained at a flow-rate of 0.8 mL per minute; the column was maintained at 40 degrees C; the DAD detector was set at 210 nm.
RESULTSThe liner range was 0.251-10.04 micrograms (r = 1.0000). An average recovery of 98.1% (n = 6) was obtained with a RSD of 1.0%.
CONCLUSIONThe method is simple, accurate and suitable for the qualify control.
