Determination of quercetin in the Herba Euphorbiae Humifusae.
- Author:
Jun-bo XING
1
;
Cai-hong SHUI
;
Yun LIU
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; Euphorbia; chemistry; Plants, Medicinal; chemistry; Quality Control; Quercetin; analysis
- From: China Journal of Chinese Materia Medica 2002;27(10):758-760
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method for determining quercetin in Herba Euphorbiae Humifusae.
METHODODS column was used with acetonitrile: 0.4% phosphoric acid (40:60) as mobile phase. The detection wavelength was at 370 nm and the flow rate was 1.0 mL.min-1.
RESULTThe linearity was obtained over the range of 0.0364-0.1274 microgram (r = 0.9999). The average recovery was 100.71% with relative standard deviation of 2.45%.
CONCLUSIONThis method can be used for quality control of Herba Euphorbiae Humifusae. It is simple, rapid and reliable.
