Determination of nucleosides in siweilingzhi mixture by HPCE.
- Author:
Jing DAI
1
;
Jing LU
;
Rui-chao LIN
;
Wen-ying LIU
Author Information
- Publication Type:Journal Article
- MeSH: Adenosine; analysis; Drugs, Chinese Herbal; administration & dosage; chemistry; isolation & purification; Electrophoresis, Capillary; methods; Fermentation; Guanosine; analysis; Reishi; chemistry
- From: China Journal of Chinese Materia Medica 2002;27(9):665-668
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for determining nucleosides (adenoside and guanoside) in Siweilingzhi Mixture by HPCE.
METHODAdenoside and guanoside were separated within 25 min using an 20 mmol.L-1 borate buffer with 30 mmol.L-1 SDS and 5% Ethanol (adjusted to pH 10.0 with sodium hydroxide solution), with an operation voltage of 10 kV, temperature of 20 degrees C and a hydrodynamic injection time of 15 s. Seperations were carried out in a fused-silica capillary 75 microns id x 57 cm (effective length 50 cm) with peak detection by direct UV at 254 nm.
RESULTRegression equation of adenoside and that of guanoside were Y = 0.0705 + 0.01707X (r = 0.9995) and Y = 0.0232 + 0.01864X (r = 0.9999) respectively. The average recovery rate was 99.22% (RSD = 3.66%) and 104.3% (RSD = 1.91%) respectively. Nine samples were determined with the method.
CONCLUSIONThe method is simple, rapid and accurate with good repeatability and it can be used to determine nucleosides.
