Expression of reconstructed BCR-ABL-pIRES-SEA plasmids in the skeletal muscles of BALB/c mice.
- Author:
Yongpeng GAO
1
;
Yanan QIN
;
Chen LIN
;
Hongxia TIAN
;
Chen CHEN
;
Yubing ZHOU
;
Yangqiu LI
Author Information
1. Department of Microbiology and Immunology, Medical College, Jinan University, Guangzhou 510632, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Enterotoxins;
genetics;
immunology;
metabolism;
Fusion Proteins, bcr-abl;
genetics;
immunology;
metabolism;
Male;
Mice;
Mice, Inbred BALB C;
Muscle, Skeletal;
metabolism;
Plasmids;
immunology;
RNA, Messenger;
genetics;
metabolism;
Recombinant Fusion Proteins;
genetics;
immunology;
metabolism;
Vaccines, DNA;
administration & dosage;
immunology
- From:
Journal of Biomedical Engineering
2012;29(3):519-523
- CountryChina
- Language:Chinese
-
Abstract:
This paper is aimed to investigate the transcription and expression of BCR-ABL-pIRES-SEA fusion gene vaccines in vivo in mice. The reconstructed plasmids (BCR-ABL-pIRES-SEA) which were developed previously in our laboratory were injected into the skeletal muscles of BALB/c mice at 14d intervals for three cycles. The transcription and expression of BCR-ABL and staphylococcal enterotoxin A (SEA) in injection site were detected using RT-PCR and immunohistological methods. The BCR-ABL/SEA mRNA and protein could be identified in the injection site of BCR-ABL-pIRES-SEA vaccinated mice. The reconstructed BCR-ABL-pIRES-SEA plasmids can effectively express gene production in the skeletal muscles of mice and have the common features of DNA vaccine.
