Tail-suspended model simulating mouse oocytes maturation inhibited with microgravity.
- Author:
Changli WU
1
;
Li LI
;
Hengxi WEI
;
Zhenfang WU
;
Qingyan JIANG
;
Shouquan ZHANG
Author Information
1. College of Animal Science, South China Agriculture University, Guangzhou 510642, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cells, Cultured;
Female;
Hindlimb Suspension;
Mice;
Oocytes;
cytology;
physiology;
Oogenesis;
physiology;
Weightlessness Simulation
- From:
Journal of Biomedical Engineering
2012;29(4):687-696
- CountryChina
- Language:Chinese
-
Abstract:
We studied the effects of simulated microgravity on mouse oocytes maturation, and analyzed whether the tail-suspended model can be applied to investigate simulated microgravity effects on reproductive processes in female mice. Mouse oocytes were cultured in vitro with microgravity simulated by a rotating wall vessel bioreactor and by tail-suspended model, and the maturation rate of the mouse oocytes in the two models were examined in vivo. The maturation rate of mouse oocytes cultured in simulated microgravity was 8.93%, and that was 72.33% in 1g gravity. In ratio, oocyte maturation rate had no significant difference between the rotational group and control group. Microgravity simulated by the tail-suspended model inhibited mouse oocytes maturation and increased the rate of oocytes abnormity. The maturation rate of tail-suspended mouse oocytes was 14.54%, which was significantly lower than that of control group. Tail-suspended model should be an ideal model to investigate simulated microgravity effects on reproductive processes of female mice.
