Research on cationic vector-mediated RNAi.
- Author:
Xu ZHANG
1
;
Huiqin DING
;
Bing WANG
;
Shaohui CUI
;
Yinan ZHAO
;
Wenshi JIN
;
Shubiao ZHANG
;
Mei JIN
Author Information
1. College of Life Science, Liaoning Normal University, Dalian 116029, China.
- Publication Type:Journal Article
- MeSH:
Cations;
chemistry;
Drug Carriers;
Genetic Vectors;
Hep G2 Cells;
Humans;
Lipids;
chemistry;
Liposomes;
chemistry;
Luciferases;
genetics;
RNA Interference;
RNA, Small Interfering;
genetics;
Transfection
- From:
Journal of Biomedical Engineering
2012;29(4):722-726
- CountryChina
- Language:Chinese
-
Abstract:
In order to study the efficiency of small interfering RNA (siRNA) transfer mediated by cationic liposome, we used luciferase siRNA to evaluate the gene silencing activity in the Hep-2 cells, which were stably transduced with a luciferase gene. The pDNA transfection was studied, and siRNA arrearage assay was conducted to determine the capability of cationic liposome with siRNA. Different concentrations of siRNA was used to silence luciferase gene' activity, and then the result was examined by microplate reader. Cell viability was analyzed after transfection by MTT assay. The results suggested that Lipofectamine 2000 could transfer the pDNA efficiently, and have strong binding capacity with siRNA. The silencing efficiency of luciferase was obtained with low concentration of siRNA. The cell viability was influenced by RNA interference (RNAi) very slightly, but the cell survival rate decreased with the increase of siRNA concentrations. It was well concluded that by optimizing the experimental conditions, cationic liposome can transfer low concentration siRNA to silence target gene's activity efficiently.
