Effects of basic fibroblast growth factor on bone marrow mesenchymal stem cell differentiation into temporomandibular joint disc cells.
- Author:
Xuelian SU
1
;
Guangjie BAO
;
Hong KANG
Author Information
1. Key Lab of Stomatology of State Ethnic Affairs Commission, Medical College, Northwest University for Nationalities, Lanzhou 730030, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Bone Marrow Cells;
cytology;
Cell Differentiation;
drug effects;
Cells, Cultured;
Collagen Type I;
biosynthesis;
Fibroblast Growth Factor 2;
pharmacology;
Glycosaminoglycans;
biosynthesis;
Goats;
Mesenchymal Stromal Cells;
cytology;
metabolism;
Temporomandibular Joint Disc;
cytology;
Tissue Engineering
- From:
Journal of Biomedical Engineering
2012;29(4):732-736
- CountryChina
- Language:Chinese
-
Abstract:
The present paper is aimed to observe the effects of basic fibroblast growth factor (bFGF) on bone marrow mesenchymal stem cell (BMSCs) differentiation. The bFGF was used to stimulate BMSCs and histology, immunohistochemistry and enzyme linked immunosorbent assay (ELISA) were used to examine the extracellular matrix produced by induced BMSCs, evaluated the feasibility of BMSCs being the seeding cells of temporomandibular joint (TMJ) disc tissue engineering. The results showed that having been induced with bFGF, the BMSCs could differentiate into fibroblast-like cells, which could synthesize GAG and collagen type I matrix. So it is feasible for BMSCs as seeding cells for engineered TMJ disc.
