Investigation of the sensitivities of two different serotypes of Streptococcus pneumoniae in C57BL/6mice with acute otitis media
10.3760/cma.j.issn.1673-0860.2013.12.012
- VernacularTitle:两种不同血清型肺炎链球菌致C57BL/6小鼠急性中耳炎的敏感性差异
- Author:
Wei WANG
1
;
Ai-E ZHOU
;
Yi-Fei HUANG
;
Yun XIANG
;
Yu-Juan HE
Author Information
1. 400016,重庆医科大学检验医学院
- Keywords:
Streptococcus pneumoniae;
Pneumococcal infections;
Otitis media;
Mice,inbred C57BL
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2013;48(12):1028-1031
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the sensitivities of two different serotypes of Streptococcus pneumoniae in acute otitis media of C57BL/6 mice.Methods The middle ear cavity of C57BL/6 mice were inoculated via intratympanic injection of Streptococcus pneumoniae TIGR4 or 6B 1 × 108 CFU,and the control group were inoculated equivalent phosphate buffered solution (PBS).The incidence of mice,development of otitis media and the middle ear lavage fluid pathological changes by HE staining,as well as cell counts and cytokine levels were investigated.Results After inoculated with Streptococcus pneumoniae TIGR4,6B and PBS,the survival rate of 6B group was significantly less than TIGR4 group and PBS control group (P ＜0.01).Inflammatory cells in the middle ear cavity were mainly neutrophils,and the inflammatory cells recruitment in TIGR4 group were more than 6B group; The levels of IL-6 and TNF-α in the middle ear lavage fluid in T1GR4 group and 6B group were significantly increased compared with PBS control group,while the TIGR4 group were significantly increased compared with 6B group ;6B group had delayed bacterial clearance in the middle ear.Conclusion The study implied that Streptococcus pneumoniae 6B had higher pathogenicity for acute otitis media in C57BL/6 mice than TIGR4.
