Specific suppression of beta-secretase gene expression by short interfering RNA in mammalian cells.
- Author:
Hai-tao HU
1
;
Wei-jiang DONG
;
Gai-feng FENG
Author Information
- Publication Type:Journal Article
- MeSH: Amyloid Precursor Protein Secretases; genetics; metabolism; Animals; Green Fluorescent Proteins; genetics; metabolism; Humans; Immunohistochemistry; Mice; Microscopy, Fluorescence; NIH 3T3 Cells; Neuroblastoma; genetics; metabolism; pathology; Plasmids; genetics; RNA Interference; RNA, Small Interfering; genetics; Recombinant Fusion Proteins; genetics; metabolism; Tumor Cells, Cultured
- From: Journal of Zhejiang University. Medical sciences 2006;35(6):622-629
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate whether short interfering RNAs(siRNAs) of beta-site APP cleaving enzyme (BACE) can inhibit the expression of BACE in mammalian cells.
METHODSThe gene of EGFP, U6 promoter and beta-secretase targeting siRNA were cloned by PCR, respectively. The PCR products were inserted into plasmid pLXSN. The interfering vector pLXSN/EGFP-U6-siBACE was transferred into SK-N-SH cells to express BACE. The inhibition effect of BACE siRNA on BACE expression was investigated by fluoroscopy and immunohistochemistry method.
RESULTThe interfering vector pLXSN/EGFP-U6-siBACE was constructed successfully. The BACE siRNA inhibited the expression of BACE in the SK-N-SH cells specifically and effectively, and the production of A beta was reduced.
CONCLUSIONBACE siRNA can inhibit the expression of BACE gene of mammalian cells.
