Stimulation by Lycium bararum polysaccharides of the maturation of dendritic cells in murine bone marrow.
- Author:
Jie ZHU
1
;
Lu-hang ZHAO
;
Zhi CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Marrow Cells; cytology; drug effects; immunology; CD11c Antigen; immunology; Cells, Cultured; Dendritic Cells; cytology; drug effects; immunology; Drugs, Chinese Herbal; pharmacology; Lipopolysaccharides; pharmacology; Lymphocyte Culture Test, Mixed; Lymphocytes; cytology; drug effects; immunology; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Phagocytosis; drug effects; immunology
- From: Journal of Zhejiang University. Medical sciences 2006;35(6):648-652
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of Lycium bararum polysaccharides (LBPs) stimulation on the maturation of murine bone marrow derived dendritic cells (BMDCs).
METHODSMurine bone marrow cells were cultured in GM-CSF and IL-4 for 5 days, then were purified with a MACS column. Respectively, BMDCs were stimulated with LBPs, LPS and RPMI1640 for 2 days. Cell phenotypes and antigens uptake by BMDCs were analyzed by flow cytometry. Cytokines released by BMDCs were detected. The antigen presenting by BMDCs was evaluated by mixed lymphocyte responses.
RESULTCompared with to the BMDCs that only subjected to RPMI 1640, the expression of I-A/I-E, CD11c and secretion of IL-12 by BMDCs stimulated with LBPs were increased, the phagocytosis of FITC-dextran by BMDCs stimulated with LBPs was impaired but the activation of proliferation of allogenic lymphocytes by BMDCs was strengthened.
CONCLUSIONLBPs promote not only the maturation of cultured murine BMDCs in vitro, but also the immune response initiation induced by BMDCs.
