Cardioprotective effect of ischemic postconditioning and preconditioning against prolonged ischemia and reperfusion induced injury in isolated rat heart.
- Author:
Ting-Mei YE
1
;
Qin GAO
;
Yan-Fang LI
;
Jue WANG
;
Qiang XIA
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Survival; In Vitro Techniques; Ischemic Preconditioning, Myocardial; methods; L-Lactate Dehydrogenase; metabolism; Male; Myocardial Ischemia; pathology; physiopathology; Myocardial Reperfusion Injury; metabolism; physiopathology; prevention & control; Myocardium; metabolism; pathology; Potassium Channels, Calcium-Activated; metabolism; Rats; Rats, Sprague-Dawley
- From: Journal of Zhejiang University. Medical sciences 2007;36(1):35-40
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the cardioprotection effect of co-treatment with ischemic postconditioning and preconditioning in ischemia/reperfusion (I/R) injury and the related mechanism.
METHODSMale Sprague-Dawley rats were used for Langendorff isolated heart perfusion. The hearts were subjected to global ischemia for 60 min followed by 120 min of reperfusion. The cardiomyocyte viability was measured by MTT-formazan method, and the cardiac injury was evaluated by the levels of lactate dehydrogenase (LDH) in the coronary effluent. Ventricular hemodynamic parameters were also measured.
RESULTIn 60 min of ischemia and 120 min of reperfusion group, ischemic postconditioning increased formazan content, reduced LDH release, but hemodynamic parameters did not improved. Co-treatment with ischemic postconditioning and preconditioning during the prolonged ischemia further improved the hemodynamic parameters. The calcium activated potassium channel antagonist paxilline attenuated the effect of co-treatment with ischemic postconditioning and preconditioning.
CONCLUSIONIschemic postconditioning and preconditioning may synergically protect myocardium from severe ischemia injury, which may be related to calcium-activated potassium channel.
