Adhesional inhibiton of polyclonal anti-porin I antibody.
- Author:
Lei ZHENG
1
;
Li-Ming RUAN
;
Hao CHENG
;
Jian-Ping CENHANG
;
Jian-Gen SHEN
;
Jie LIN
;
Hang ZHANG
;
Yun-Bin YAO
;
Qi WANG
;
Da-Fang CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Monoclonal; pharmacology; Bacterial Adhesion; drug effects; Epithelium; drug effects; microbiology; Female; Glutathione Transferase; biosynthesis; genetics; Gonorrhea; microbiology; prevention & control; Mice; Mice, Inbred BALB C; Neisseria gonorrhoeae; drug effects; physiology; Porins; biosynthesis; genetics; immunology; Rabbits; Recombinant Fusion Proteins; biosynthesis; genetics; immunology; Urogenital System; drug effects; microbiology
- From: Journal of Zhejiang University. Medical sciences 2007;36(1):78-83
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the blockness effects of purified polyclonal anti-porin I antibody on N. gonorrhoeae adherence to genitourinary tract epithelia of BALB/c mouse.
METHODSPolyclonal anti GST-PI antibody was generated by immunizing rabbit with GST-PI fusion protein which was constructed and expressed by ourselves. The purified immunoglobulin G was obtained by ammonium sulphate deposition and DEAE cellulose chromatography. Mice model of gonorrhea was established. In order to evaluate the effects of PI-IgG on gonococcus adhesion to vagina mucus, the macroscopic and pathological assessing as well as gonococcus culture was employed after gonococcus challenge on PI-IgG immunized mice.
RESULTNo pus and pathological inflammation were observed on mice vagina mucus treated with 1 mg/ml PI-IgG 3 hours before gonococcus challenge. Gonococcus could not be detected in the smears and washing solutions from vagina. Pathological inflammation was found in mice treated with anti PI-IgG, in which the concentrations were lower than 1 mg/ml or the treated time was longer than 3 hours prior to gonococcus challenge.
CONCLUSIONThe purified anti PI-IgG can effectively inhibit the adherence and infection of gonococci to genitourinary tract epithelia of BALB/c mice. In addition, the blocking duration of anti PI-IgG is associated with antibody concentration.