Effects of cysteinyl receptor agonist and antagonists on rat primary cortical neurons.
- Author:
Xin HU
1
;
Qiu-Fu GE
;
Wei-Ping ZHANG
;
Er-Qing WEI
Author Information
- Publication Type:Journal Article
- MeSH: Acetates; pharmacology; Animals; Animals, Newborn; Calcium; metabolism; Cell Hypoxia; Cell Survival; drug effects; Cells, Cultured; Cerebral Cortex; cytology; Chromones; pharmacology; Glucose; pharmacology; Leukotriene Antagonists; pharmacology; Leukotriene D4; pharmacology; Neurons; cytology; drug effects; metabolism; Quinolines; pharmacology; Rats; Receptors, Leukotriene; agonists
- From: Journal of Zhejiang University. Medical sciences 2007;36(2):117-122
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the effect of cysteinyl receptor agonist leukotriene D(4) (LTD(4)) and its antagonists on rat primary neurons.
METHODSIn the primarily cultured rat cortical neurons, the neuron injury was evaluated by measuring intracellular calcium, 3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyl tetrazolium bromide (MTT) reduction, and propidium iodide (PI) and Hoechst 33258 staining. The in vitro ischemic injury was induced by oxygen-glucose deprivation (OGD) for 1.5 h and reperfusion for 24 h.
RESULTLTD(4) (0.01-1 micromol/L) did not induce the elevation of intracellular calcium, neuron viability changes and neuron death. OGD-induced injury was not significantly ameliorated by the CysLT(1) receptor antagonists, pranlukast (0.2-10 micromol/L) and montelukast (0.2-10 micromol/L), as well as by the CysLT(1)/CysLT(2) receptor non-selective antagonist, BAY u9773 (0.02-1 micromol/L).
CONCLUSIONNeither agonist nor antagonists of cysteinyl receptors have the effects on the viability and ischemic-like injury in rat primary neurons.
