Rat microsomal glutathione S-transferase 1 alters cytotoxic effects of chlorambucil on PC-3, K562, HepG2 and P388D1 cell lines.
- Author:
Zhe CHEN
1
;
Zi-qi YE
;
Qiang SHI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antineoplastic Agents, Alkylating; metabolism; pharmacology; Apoptosis; drug effects; Cell Line, Tumor; Cell Survival; drug effects; Chlorambucil; metabolism; pharmacology; Dose-Response Relationship, Drug; Glutathione Transferase; isolation & purification; metabolism; Humans; K562 Cells; Microsomes, Liver; enzymology; Rats; Rats, Sprague-Dawley
- From: Journal of Zhejiang University. Medical sciences 2007;36(3):236-240
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the possible association between activation of rat microsomal glutathione S-transferase 1 (mGST1) and chlorambucil toxicity on selected cancer cell lines.
METHODSHepatic microsomes were prepared from male Sprague-Dawley rats and washed to remove cytosolic contamination. mGST1 was purified and its activity was measured. PC-3, K562, HepG2 and P388D1 cell lines were exposed to chlorambucil (CHB) alone or to CHB with mGST1 at concentrations of 0 ~ 100 micromol/L for 8, 24, 48, 72 h. Cytotoxic effects of CHB were determined by cell growth inhibition (MTT assay), mitochondrial transmembrane potential (DeltaPsim), and fluorescence morphological examination (AO/EB staining).
RESULTSThe decreased cytotoxic effects of CHB on the cell lines altered by mGST1 were demonstrated in concentration- and time-dependant manners. The CHB-induced apoptosis on PC-3 and K562 cell lines altered by mGST1 was confirmed using DeltaPsim examination, JC-1 or AO/EB staining.
CONCLUSIONmGST1 can reduce the cytotoxic effects of CHB in selected cancer cell lines.
