DMF induces apoptosis in human androgen-independent prostate cancer PC3 cells in vitro.
- Author:
Yan-fen FANG
1
;
Tao LIU
;
Ji CAO
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; pharmacology; Apoptosis; drug effects; Blotting, Western; Caspase 3; metabolism; Cell Line, Tumor; Cell Proliferation; drug effects; Chlorobenzenes; pharmacology; Dose-Response Relationship, Drug; Enzyme Activation; drug effects; Flavonoids; pharmacology; Flow Cytometry; Growth Inhibitors; pharmacology; Humans; Male; Membrane Potential, Mitochondrial; drug effects; Piperidines; pharmacology; Prostatic Neoplasms; metabolism; pathology; Proto-Oncogene Proteins c-bcl-2; metabolism; bcl-2-Associated X Protein; metabolism; p38 Mitogen-Activated Protein Kinases; metabolism
- From: Journal of Zhejiang University. Medical sciences 2007;36(3):255-260
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the antiproliferative activity of 3-(2-chlorophenyl)-1-(2-hydroxy-4, 6-dimethoxy-3-((ethyl(methyl) amino) methyl) phenyl) prop-2-en-1-one (DMF) against human androgen-independent prostate cancer PC3 cells in vitro and its underlying mechanisms.
METHODSThe cytotoxic effect of DMF on PC3 cells was measured by MTT assay. Induction of apoptosis was assessed by propidium iodide staining and flow cytometric analysis. Changes of mitochondrial membrane potential (DeltaPsim) were detected by JC-1 staining. The levels of apoptosis related proteins were analyzed by Western blot.
RESULTSDMF exhibited high efficiency on cell growth inhibition in PC3 cells with an IC50 value of (9.5 +/- 0.2)micromol/L. Flow cytometric analysis indicated that DMF could induce apoptosis in PC3 cells. A significant decrease of mitochondrial membrane potential was observed in PC3 cells treated with DMF, which was in a time- and dose-dependent manner. The results of Western blot indicated that DMF induced the activation of caspase-3, increased the ratio of Bax/Bcl-2 and downregulated the expression of phosphate-p38.
CONCLUSIONDMF is a potential compound against PC3 cells and the mitochondrial pathway might be involved in DMF-induced apoptosis in PC3 cells.
