Dihydroartemisinin inhibits proliferation and induces apoptosis of rat glioma C6 cells.
- Author:
Zhen-qiu MA
1
;
Xiao-jia HUANG
;
Wei-ping ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antineoplastic Agents, Phytogenic; pharmacology; Apoptosis; drug effects; Artemisinins; pharmacology; Brain Neoplasms; metabolism; pathology; Cell Line, Tumor; Cell Proliferation; drug effects; Glioma; metabolism; pathology; Inhibitory Concentration 50; Rats; Reactive Oxygen Species; metabolism; Sesquiterpenes; pharmacology; Time Factors
- From: Journal of Zhejiang University. Medical sciences 2007;36(3):267-272
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the effects of dihydroartemisinin (DHA) on the proliferation and apoptosis of rat glioma C6 cells.
METHODSDHA (1~ 125 micromol/L) was added into the cultured C6 cells and incubated for 24, 48 and 72 h. The cell proliferation and viability were determined by trypan blue exclusion assay and 3-(4, 5-dimethylthiazol-2yl)-2, 5 diphenyl tetrazolium bromide (MTT) reduction assay. The apoptosis was detected by Hoechst 33342 staining. The intracellular reactive oxygen species (ROS) was measured by H(2)DCFDA oxidative reaction.
RESULTSDHA 5 ~ 125 micromol/L inhibited the proliferation of C6 cells in concentration- and time-dependent manners, the IC50 at 48 h was 23.4 micromol/L. DHA 5 ~ 25 micromol/L induced C6 cell apoptosis (P<0.05), and 5 ~ 125 micromol/L increased the intracellular ROS (P<0.01).
CONCLUSIONDHA inhibits the proliferation and induces the apoptosis of C6 cells; its cytotoxic effect may result from the increase of intercellular ROS.
