Interaction between a novel centrosomal protein TACP1 and mitotic kinase Nek2A.
- Author:
Yuan-yuan ZHU
1
;
Jian-ping LAN
;
Jian YU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Centrosome; metabolism; Escherichia coli; genetics; Fluorescent Antibody Technique; HeLa Cells; Humans; Immunoprecipitation; Mitosis; NIMA-Related Kinases; Protein Binding; Protein-Serine-Threonine Kinases; genetics; metabolism; Recombinant Proteins; genetics; metabolism; Telomere-Binding Proteins; genetics; metabolism; Transfection
- From: Journal of Zhejiang University. Medical sciences 2007;36(4):337-342
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study interaction between a novel centrosomal protein TACP1 and mitotic kinase Nek2A.
METHODSNek2A305-446 protein was expressed and purified in E.coli and TACP1 protein was expressed in transfected 293T cells. Pull-down assay was used to examine the interaction between Nek2A305-446 and TACP1. TACP1 and Nek2A complex was tested by co-immunoprecipitation assay with polyclonal anti-TACP1 antibody. The localization of those two proteins in Hela cells was verified by immunofluorescence.
RESULTSTACP1 was pulled down by Nek2A305-446 protein but not by GST control. Nek2A was co-precipitated with TACP1 protein by polyclonal anti-TACP1 antibody but not by pre-immunization serum. The Immunofluorescence test showed that these two proteins formed a complex at centrosome during mitosis.
CONCLUSIONCentrosomal protein TACP1 is a novel interacting protein with Nek2A, both of which are localized in centrosome during mitosis.
