Screening for metronidazole-resistance associated gene fragments of Helicobacter pylori by suppression subtractive hybridization.
- Author:
Ai-qing LI
1
;
Ning DAI
;
Jie YAN
;
Yong-liang ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Anti-Infective Agents; pharmacology; Cloning, Molecular; DNA, Bacterial; chemistry; genetics; Drug Resistance, Bacterial; genetics; Helicobacter pylori; drug effects; genetics; Humans; Metronidazole; pharmacology; Nucleic Acid Hybridization; methods; Sequence Analysis, DNA
- From: Journal of Zhejiang University. Medical sciences 2007;36(5):465-469
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen metronidazole (MTZ) -resistance associated gene fragments of Helicobacter pylori (H.pylori) by suppression subtractive hybridization(SSH).
METHODSThe suppression subtractive hybridization (SSH) was used to screen the different DNA fragments between MTZ-resistant and -susceptible clinical strains of H.pylori. The resistant strains specific gene fragments were obtained by SSH and identified by dot blotting.
RESULTAmong the 120 subtractive colonies which were randomly chosen, 37 DNA fragments were different (>or=2 times) in DNA-copy numbers between resistant and susceptible strains and 17 of them were significantly different (>or=3 times). These 17 DNA fragments were sequenced subsequently. Ten of them were new sequences and the other 7 were duplicated sequences. These sequences represented respectively: depeptide ABC transporter periplasmic dipeptide-binding protein (dppA), permease protein (dppB), et al.
CONCLUSIONGene fragments specific to MTZ-resistant H. pylori strains were obtained by SSH and these genes may associated with MTZ-resistance of H.pylori.
