Expression of cyclin D1 and p27kip1 in renal cell carcinoma and its significance.
- Author:
Jian-rong GE
1
;
Guo-ping REN
;
Hang-ping YAO
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Aged, 80 and over; Blotting, Western; Carcinoma, Renal Cell; genetics; metabolism; pathology; Cyclin D1; biosynthesis; genetics; Cyclin-Dependent Kinase Inhibitor p27; biosynthesis; genetics; Female; Gene Expression Regulation, Neoplastic; Humans; Kidney Neoplasms; genetics; metabolism; pathology; Male; Middle Aged; RNA, Messenger; biosynthesis; genetics; Reverse Transcriptase Polymerase Chain Reaction
- From: Journal of Zhejiang University. Medical sciences 2007;36(5):483-487
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of cyclin D1 and p27(kip1) in the occurrence and development of conventional renal cell carcinoma(CRCC).
METHODSRT-PCR and Western-blot were used to detect mRNA and protein contents of cyclin D1 and p27(kip1) in 25 CRCCs and 10 normal renal tissue distant to tumor. Immunohistochemistry was used to investigate the expression of cyclin D1 and p27(kip1) in pathological tissue sections of 76 CRCCs. The relationship between those index and clinicopathological parameters was analyzed statistically.
RESULTIn CRCC, the expression of cyclin D1 was higher than that of the control group. The higher cyclin D1 content was related to big tumor size (P<0.05); The expression of p27(kip1) was lower than that of the control group, and the lower p27(kip1) was related to higher nuclear grade and TNM stage (P<0.01). The 5-year cumulative survival rate of the p27(kip1) high expression group is longer than that of the low group (P<0.01).
CONCLUSIONThe excessive expression of cyclin D1 and lower expression of p27(kip1) play an important role in the carcinogenesis of CRCC. The lower expression of p27(kip1) may affect the progression of the tumors. The detection of p27(kip1) may be as a reference marker in the prognosis of CRCC.
