Effect of ginkgolide B on the function of rat aorta smooth cells and U937 cells stimulated by oxLDL.
- Author:
Yu-Jia MAO
1
;
Lin WANG
;
Wen-Jie WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Aorta, Thoracic; cytology; Cell Proliferation; drug effects; Cells, Cultured; Chemokine CCL2; biosynthesis; genetics; Diterpenes; isolation & purification; pharmacology; Dose-Response Relationship, Drug; Ginkgo biloba; chemistry; Ginkgolides; Humans; I-kappa B Proteins; metabolism; Interleukin-8; biosynthesis; genetics; Lactones; isolation & purification; pharmacology; Lipoproteins, LDL; pharmacology; Male; Muscle, Smooth, Vascular; cytology; Myocytes, Smooth Muscle; drug effects; metabolism; Plants, Medicinal; chemistry; Platelet Activating Factor; antagonists & inhibitors; RNA, Messenger; biosynthesis; genetics; Rats; Rats, Wistar; Synaptotagmin I; metabolism; U937 Cells
- From: Acta Pharmaceutica Sinica 2006;41(1):36-40
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the effect of ginkgolide B on the proliferation of VSMC and the secretion of chemokines by U937 cells stimulated by oxLDL or PAF. In addition, to analyze whether the effect of oxLDL is mediated through PAF receptor.
METHODSUsing 3H-Tdr incorporation assay, the proliferation of VSMC was measured. The protein and mRNA level of MCP-1 and IL-8 in U937 cells were determined by RT-PCR and ELISA. Using Western blotting the p65 and IkappaB was quantified. The binding of oxLDL to U937 cell was measured by a radio-ligand binding assay of 3H-PAF.
RESULTSGinkgolide B inhibited, in dose-dependent manner, the proliferation of VSMC and the secretion of chemokines by U937 cells stimulated by oxLDL, and inhibited the oxLDL-induced p65 activation and depletion of IKappaB. oxLDL inhibited PAF binding to U937 cells.
CONCLUSIONGinkgolide B, as a PAF antagonist, possesses the effect of inhibiting the proliferation of VSMC and the secretion of chemokines by U937 cells stimulated by oxLDL in vitro. The effect of oxLDL is, at least in part, mediated through PAF receptor.
