Effect of Angelica sinensis polysaccharide fraction AP-3 on IL-2 and IFN-gamma induction.
- Author:
Tie-Hong YANG
1
;
Min JIA
;
Qi-Bing MEI
Author Information
- Publication Type:Journal Article
- MeSH: Angelica sinensis; chemistry; Animals; CD4-Positive T-Lymphocytes; cytology; Cell Proliferation; drug effects; Cells, Cultured; Female; Interferon-gamma; biosynthesis; genetics; Interleukin-2; biosynthesis; genetics; Lymphocyte Activation; drug effects; Male; Mice; Mice, Inbred BALB C; Plants, Medicinal; chemistry; Polysaccharides; isolation & purification; pharmacology; RNA, Messenger; biosynthesis; genetics; Spleen; cytology; metabolism
- From: Acta Pharmaceutica Sinica 2006;41(1):54-57
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the effects of Angelica sinensis polysaccharide fraction AP-3 on IL-2 and IFN-gamma induction and its further immunomodulatory feature.
METHODSThe percentage of CD4+ lymphocyte was detected by flow cytometric method, the production of IL-2 and IFN-gamma in cell culture supernatant were determined by ELISA, mRNA expressions of IL-2 and IFN-gamma cytokines were detected by RT-PCR.
RESULTSAt the range of 0. 6 - 2 micromol x L(-1), AP-3 significantly enhanced the percentage of CD4+ lymphocytes in total splenocytes. At the range of 2 - 6 micromol x L(-1), the treatment of AP-3 augmented both productions of IL-2 in cell culture supernatant and cell IL-2 mRNA transcription level in a time and dose dependent manner. While in the case of IFN-gamma, AP-3 stimulated at early time after exposure but down-regulated thereafter.
CONCLUSIONAngelica sinensis polysaccharide could regulate the immune response through upregulating IL-2, IFN-gamma expression and activating Th1 cell.
