HPLC-MSn analysis of trigonelline and its metabolites in rat urine.
- Author:
Yong CHEN
1
;
Shao-lin SHEN
;
Huai-xia CHEN
;
Jun PAN
;
Feng-mei HAN
Author Information
- Publication Type:Journal Article
- MeSH: Alkaloids; chemistry; isolation & purification; metabolism; Animals; Chromatography, High Pressure Liquid; methods; Hypoglycemic Agents; chemistry; isolation & purification; metabolism; Male; Plants, Medicinal; chemistry; Rats; Rats, Wistar; Sensitivity and Specificity; Spectrometry, Mass, Electrospray Ionization; methods; Trigonella; chemistry
- From: Acta Pharmaceutica Sinica 2006;41(3):216-220
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo establish a rapid and sensitive LC-MSn method for the identification of trigonelline and its main metabolites in rat urine.
METHODSAfter optimizing the detection conditions of LC-MSn chromatography and mass spectrometry using trigonelline, its ionization and cleavage in ESI-MS and ESI-MSn modes were summarized, then serving as the basis for the metabolite analysis of trigonelline in rat urine. The 0-48 h urine samples of rats were collected after iv 8 mg x kg(-1) trigonelline, then, the samples were purified through C18 solid-phase extraction cartridge. The purified samples were analyzed by LC-MSn.
RESULTSThe structures of trigonelline metabolites were elucidated according to the changes of the molecular weights of the metabolites (deltaM) and their cleavage pattern in ESI-ITMSn. As a result, two phase I metabolites and the parent drug were identified existing in rat urine, and two phase II metabolites were identified.
CONCLUSIONThe LC-MSn method is rapid and high sensitive and specific, it is suitable for the identification of trigonelline and its metabolites in rat urine.
