Induction of apoptosis of tumor multidrug resistance cell by uvarigrin and its mechanism.
- Author:
Yan-fang LI
1
;
Yong-ju LIANG
;
Zhi SHI
;
Li-ming CHEN
;
Yan DING
;
Li-wu FU
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents, Phytogenic; administration & dosage; isolation & purification; pharmacology; Apoptosis; drug effects; Caspase 9; metabolism; Cell Proliferation; drug effects; Dose-Response Relationship, Drug; Drug Resistance, Multiple; Drug Resistance, Neoplasm; Furans; administration & dosage; isolation & purification; pharmacology; Humans; KB Cells; Lactones; administration & dosage; isolation & purification; pharmacology; Membrane Potentials; drug effects; Mitochondria; physiology; Plants, Medicinal; chemistry; Reactive Oxygen Species; metabolism; Uvaria; chemistry
- From: Acta Pharmaceutica Sinica 2006;41(3):252-256
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the effect of uvarigrin on mitochondrial dependent pathway during the apoptosis induced by it in MDR KBv200 cells and their parental sensitive KB cells.
METHODSMTT assay was used to detect the cytotoxic effect of uvarigrin on KBv200 and KB cells. Annexin V FITC staining identified uvarigrin-induced apoptosis in KBv200 and KB cells. These cells underwent incubation with DCFH-DA, or DiOC6, followed by flowcytometry for the measurement of reactive oxygen species (ROS) and mitochondrial membrane potential (deltapsim), respectively. The Western blotting analysis was performed on Caspase-9 activation.
RESULTSUvarigrin inhibited the growth of KBv200 cells and KB cells in vitro. Most of the uvarigrin-induced cells death was found to be due to apoptosis, as determined by Annexin V FITC staining. During the apoptosis, the level of ROS increased while the level of deltapsim decreased in a time-dependent manner. Uvarigrin triggered Caspase-9 activation.
CONCLUSIONUvarigrin induced apoptosis in KBv200 cells and KB cells probably through a mitochondria-dependent pathway.
