Anticoagulant property of a semi-synthesized sodium beta-1,4-glucan sulfate.
- Author:
Zhao-mei WANG
1
;
Lin LI
;
Bing LI
;
Si-yuan GUO
Author Information
- Publication Type:Journal Article
- MeSH: Anticoagulants; administration & dosage; pharmacology; Antithrombin III; pharmacology; Blood Coagulation; drug effects; Dose-Response Relationship, Drug; Factor Xa; metabolism; Glucans; administration & dosage; pharmacology; Heparin; pharmacology; Humans; Partial Thromboplastin Time; Prothrombin; metabolism; Prothrombin Time; Thrombin Time
- From: Acta Pharmaceutica Sinica 2006;41(4):323-327
- CountryChina
- Language:English
-
Abstract:
AIMTo investigate the anticoagulant efficacy and mechanism of a semi-synthesized sodium beta-1,4-glucan sulfate (Na-MCS).
METHODSAnticoagulant activity was evaluated by means of coagulation assays in comparison with heparin. The anticoagulant mechanism of Na-MCS was disclosed by inhibitory analysis of the activities of coagulation factors using chromogenic substrates.
RESULTS0.6 microg x mL(-1) Na-MCS could significantly prolong APTT and TT, but has less effect on PT at an even higher concentration. The dosage of Na-MCS required to double APTT of normal human plasma was 0.7 microg x mL(-1), lower than that of heparin with the activity of 150 u x mg(-1).
CONCLUSIONNa-MCS represented a potent anticoagulation activity in vitro, which matched the efficacy of heparin in a certain range of concentrations. Na-MCS exhibited anticoagulant activity due to inhibition of the coagulation factors IIa and Xa by the mediation of anti-thrombin AT-III.
