mRNA expression of voltage-dependent potassium channels in the brain of rats after middle cerebral artery occlusion.
- Author:
Hai-xia ZHANG
1
;
Zheng-bin LI
;
Xiao-liang WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain; metabolism; Infarction, Middle Cerebral Artery; metabolism; Kv1.4 Potassium Channel; biosynthesis; genetics; Kv1.5 Potassium Channel; biosynthesis; genetics; Male; Potassium Channels, Voltage-Gated; biosynthesis; genetics; RNA, Messenger; biosynthesis; genetics; Rats; Rats, Wistar; Shab Potassium Channels; biosynthesis; genetics; Shal Potassium Channels; biosynthesis; genetics; Up-Regulation
- From: Acta Pharmaceutica Sinica 2006;41(4):328-332
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the mRNA expression changes in the brain of rats after middle cerebral artery occlusion.
METHODSMiddle cerebral artery occlusion was used to induce ischemia in rat brain. The mRNA expression of voltage-dependent potassium channel subtypes, including Kv1.4, Kv1.5, Kv2.1 and Kv4.2, were detected in rat hippocampus and cortex by RT-PCR.
RESULTSMiddle cerebral artery occlusion induced a significant neurological injury in rats. After ischemia 2 h, the mRNA of Kv1.4, Kv2.1 and Kv4.2 in hippocampus increased by 50%, 67% and 90% , respectively. And the mRNA of Kv1.4 and Kv4.2 maintained at a high level in hippocampus after ischemia 24 h. In cortex, the mRNA level of all the four subtypes were not changed significantly after ischemia 2 h, but the mRNA of Kv2.1 and Kv4.2 increased by 70% and 62% after ischemia 24 h, respectively.
CONCLUSIONThe mRNA expression levels of voltage-dependent potassium channels were up-regulated in rat hippocampus and cortex after middle cerebral artery occlusion.
