Determination of alkaloids in Radix Aconiti Lateralis Preparata by RP-ion-pair HPLC.
- Author:
Xiu-xiu LIU
1
;
Ruo-bing CHAO
Author Information
- Publication Type:Journal Article
- MeSH: Aconitine; analogs & derivatives; analysis; isolation & purification; Aconitum; chemistry; Alkaloids; analysis; isolation & purification; Chromatography, High Pressure Liquid; methods; Plant Roots; chemistry; Plants, Medicinal; chemistry
- From: Acta Pharmaceutica Sinica 2006;41(4):365-369
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo separate and quantitatively determine six alkaloids: aconitine, mesaconitine, hypaconitine, beiwutine, benzoylaconine and benzoylmesaconine in the Chinese traditional medicine Radix Aconiti Lateralis Preparata (Fuzi).
METHODSA RP-ion-pair HPLC method was established. An AichromBond-1 C18 column was used at a column-temperature of 35 degrees C. The mobile phase was CH3CN5 mmol x L(-1) NaH2PO4(50:50) containing 7 mmol x L(-1) SDS at a flow-rate of 1.0 mL x min(-1). The detector was set at UV 235 nm.
RESULTSThese six alkaloids can be completely separated and determined quantitatively.
CONCLUSIONThis method is accurate and suitable for the determination of six alkaloids in Fuzi.
