Analysis of anisodine and its metabolites in rat plasma by liquid chromatography-tandem mass spectrometry.
- Author:
Huai-Xia CHEN
1
;
Peng DU
;
Feng-Mei HAN
;
Yong CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Administration, Oral; Animals; Chromatography, Liquid; methods; Plants, Medicinal; chemistry; Rats; Rats, Wistar; Scopolamine Derivatives; isolation & purification; metabolism; Sensitivity and Specificity; Solanaceae; chemistry; Tandem Mass Spectrometry; methods
- From: Acta Pharmaceutica Sinica 2006;41(6):518-521
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo identify anisodine and its metabolites in rat plasma after ingestion of anisodine by combining liquid chromatography and tandem mass spectrometry (LC-MS(n)).
METHODSPlasma samples from rats after a single orally administration of 20 mg anisodine were added with methanol to precipitate protein. Then, it was analyzed by LC-MS(n). Identification and structural elucidation of the metabolites were performed by comparing their changes in molecular masses, retention-times and full scan MS(n) spectra with those of the parent drug and blank plasma.
RESULTSThe results revealed that the parent drug and its four metabolites (norscopine, scopine, hydroxyanisodine, N-oxide anisodine) existed in rat plasma.
CONCLUSIONThis method is sensitive, rapid, simple, and it is suitable for the rapid identification of drug and its metabolits.
