Quantitative determination of the (E) - and (Z) -diastereomers of resveratrol and resveratrol glucoside in the roots of Polygonum cuspidatum by HPLC and elementary study on their fluorescence.
- Author:
Bo WU
1
;
Han-Jun ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Fallopia japonica; chemistry; Fluorescence; Glucosides; analysis; chemistry; Plant Roots; chemistry; Plants, Medicinal; chemistry; Reproducibility of Results; Sensitivity and Specificity; Stereoisomerism; Stilbenes; analysis; chemistry
- From: Acta Pharmaceutica Sinica 2006;41(6):522-526
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo establish an optimal HPLC method for the determination of the (E)- and (Z)-diastereomers of resveratrol and resveratrol glucoside from the roots of Polygonum cuspidatum.
METHODSThe determination was conducted by using reversed-phase high liquid chromatography. Nucleodur 100-5 C18 column (250 mm x 4.6 mm ID, 5 microm), and a mobile phase program of gradient elution with isopropyl alcohol and water at a flow rate of 0.6 mL x min(-1) was employed. The fluorescence detection wavelengths were: lambda(ex) 334 nm, lambda(em) 404 nm.
RESULTSA good linear relationship was obtained under the optimum condition. The average recoveries were 96.7%, 99.1% for the (E)-diastereomers of resveratrol and resveratrol glucoside, 91.1%, 93.7% for the (Z)-diastereomers of resveratrol and resveratrol glucoside, respectively. The RSD of E-resveratrol and its glucoside were 1.34% and 0.72%, respectively. The RSD of Z-resveratrol and its glucoside were 1.27% and 2.08%, respectively.
CONCLUSIONThis method is accurate and reliable for the quantity analysis of the (E)- and (Z)-diastereomers of resveratrol and resveratrol glucoside in the roots of Polygonum cuspidatum.
