Effect of hyaluronic acid chitosan-based microemulsion on the permeability of blood brain barrier in mice.
- Author:
Jing YAO
1
;
Jian-ping ZHOU
;
Qi-neng PING
;
Yun LU
;
Li YU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blood-Brain Barrier; drug effects; metabolism; Brain; metabolism; Chitosan; chemistry; pharmacology; Dose-Response Relationship, Drug; Emulsions; Evans Blue; chemistry; pharmacokinetics; Female; Hyaluronic Acid; chemistry; pharmacokinetics; pharmacology; Male; Mice; Particle Size; Tissue Distribution
- From: Acta Pharmaceutica Sinica 2006;41(7):615-618
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the effect of hyaluronic acid chitosan-based microemulsion (HAC-ME) on the permeability of blood brain barrier( BBB) by using Evans blue (EB) as the indicator.
METHODSA formamide extraction-ultraviolet spectrophotometry method was employed to determine the concentrations of EB in each of the tissues. The in vivo distribution of HAC-ME groups containing EB in mice and the fluorescence intensity and diffusion domain of brain slices were all studied.
RESULTSContrasting to the common microemulsion (ME), HAC-ME at the lower concentration of HAC (<5 mg x mL(-1)) could further improve the transporting of EB across the BBB while EB concentration in other tissues decreased, and Tmax was delayed about 30 min.
CONCLUSIONHAC-ME could facilitate the transporting of EB across the BBB and it was concentration dependent. While the brain targeting absorptive capability of HAC-ME was enhanced.
