Molecular Characterization of the L Segment of Hantann Virus, Strain Howang.
- Author:
Yong Kyu CHU
1
;
Dae Yong SONG
;
Hyung Mo KOO
;
Ho Wang LEE
Author Information
1. Department of Virology, Asan Institute for Life Sciences, Seoul 138-040, Korea.
- Publication Type:Original Article
- Keywords:
Hantavirus;
Hantaan virus strain Howang;
L segment;
Nucleotide sequence
- MeSH:
Amino Acid Sequence;
Amino Acids;
Base Sequence;
Genome;
Hantaan virus;
Hantavirus;
Hemorrhagic Fever with Renal Syndrome;
Molecular Weight;
Nucleotides;
Open Reading Frames;
Polymerase Chain Reaction;
Puumala virus;
RNA;
RNA Replicase;
RNA-Directed DNA Polymerase;
Seoul virus;
Sin Nombre virus
- From:Journal of the Korean Society of Virology
1999;29(3):155-163
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Hantaan virus (HTNV), the etiologic agent of hemorrhagic fever with renal syndrome (HFRS), belongs to the genus Hantavirus, and has three single negative straded RNA genome segments. HTNV strain Howang isolated from the blood of severe case of Korean HFRS is more virulent than HTNV 76/118 and the M and S genome segments' nucleotide sequence of Howang strain showed 93.5% and 94% homology to each segment of HTNV 76/118. We have obtained 6533 nucleotides long sequence of the L genome segment of Howang strain using reverse transcriptase in conjunction with PCR amplification and compared to other hantaviruses. The messenger sense of the L segment contains one long single long open reading frame of 2151 amino acids, which encodes a deduced RNA dependent RNA polymerase of 246.4 kDa caculated molecular weight protein. The nucleotide sequence of the Lsegment of Howang strain shows 93%, 74%, 66%,65% homology to HTNV 76/118, Seoul virus 80/39, Puumala virus Hallnas B1 and Sin Nombre virus, respectively. The amino acid sequence of the L segment of Howang strain shows 99%, 85%, 68%, 68% homology to HTNV 76/118, Seoul virus 80/39, Puumala virus Hallnas B1 and Sin Nombre virus, respectively.
