Antisense DNMT1 gene fragment in the sensitivity change of SMMC-7721 cells to tumor necrosis factor related apoptosis inducing ligand and its mechanism.
- Author:
Xiao-an LI
1
;
Dian-chun FANG
;
Hong ZHANG
;
Jin-liang YANG
;
Pei-ren SI
;
Ru-gang ZHANG
;
Liu-qin YANG
Author Information
- Publication Type:Journal Article
- MeSH: Antisense Elements (Genetics); genetics; Apoptosis; drug effects; Apoptosis Regulatory Proteins; Carrier Proteins; analysis; Cell Line, Tumor; DNA (Cytosine-5-)-Methyltransferase 1; DNA (Cytosine-5-)-Methyltransferases; antagonists & inhibitors; genetics; Flow Cytometry; Humans; Liver Neoplasms; metabolism; pathology; Membrane Glycoproteins; pharmacology; Proto-Oncogene Proteins; analysis; Proto-Oncogene Proteins c-bcl-2; TNF-Related Apoptosis-Inducing Ligand; Tumor Necrosis Factor-alpha; pharmacology; bcl-2-Associated X Protein; bcl-Associated Death Protein
- From: Chinese Journal of Oncology 2003;25(6):538-541
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the sensitivity change of SMMC-7721 cells transfected with antisense DNMT1 gene fragment to tumor necrosis factor related apoptosis inducing ligand (TRAIL) and its mechanism.
METHODSCell survival rate was measured by trypan blue, apoptosis rate by TUNEL method and the expression of bcl-2, bax and bad by flow cytometry.
RESULTSCell survival rate of SMMC-7721 cells transfected with antisense DNMT1 gene fragment was markedly lower than that transfected with sense DNMT1 gene fragment or empty vector (P < 0.05 and 0.01), but the apoptosis rate was on the contrary (P < 0.05 or 0.01). The expression of bax and bad (especially the former), but not bcl-2 of SMMC-7721 cells transfected with antisense DNMT1 gene fragment was markedly higher than those of SMMC-7721 cells transfected with sense DNMT1 gene fragment or empty vector.
CONCLUSIONThe sensitivity of SMMC-7721 cells to TRAIL can be enhanced by the transfection of antisense DNMT1 gene fragment, which may be related to the increase of bax and bad expression.
