Contribution of eukaryotic initiation factor-4E inhibition to heparanase expression and activity in human colon adenocarcinoma cell line LS-174T.
- Author:
Yu-jie YANG
1
;
Ya-li ZHANG
;
Zhuo-sheng LAI
;
Ji-de WANG
;
Bao-ping WU
;
Ya-dong WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; enzymology; pathology; Cell Line, Tumor; Colonic Neoplasms; enzymology; pathology; Eukaryotic Initiation Factor-4E; antagonists & inhibitors; genetics; physiology; Glucuronidase; metabolism; Humans; Neoplasm Invasiveness
- From: Chinese Journal of Oncology 2003;25(6):542-545
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine whether the eukaryotic initiation factor-4E (eIF-4E) is involved in the cap-dependent translational regulation of heparanase and study the correlation between heparanase expression and metastatic potential of LS-174T cells.
METHODSThe protein and mRNA levels of inhibited eIF-4E were tested by Western blot and RT-PCR. Heparanase activity was defined as the ability to degrade high molecular weight (40-100 000) radiolabeled ((35)S) heparan sulfate (HS) substrate into low molecular weight (5-15 000) HS fragments. The invasive potential of tumor cells in vitro was observed by Matrigel invasion assay system.
RESULTSThe 20-mer antisense oligonucleotide (asODN) against eIF-4E specifically and significantly inhibited eIF-4E expression at both transcriptional and translational levels. The expression and the activity of heparanase were effectively lowered, which further decreased the invasive potential of LS-174T.
CONCLUSIONeIF-4E, probably being involved in translational regulation of heparanase in colon adenocarcinoma cell line LS-174T, can be a particularly interesting target for heparanase regulation, based on of its critical function.
