The expression and possible function of RhoA in human gastric cancer cell lines.
- Author:
Na LIU
1
;
Feng BI
;
Yang-lin PAN
;
Yan XUE
;
Xing ZHANG
;
Yong-quan SHI
;
Yu-mei ZHANG
;
Jing-ping DU
;
Dai-ming FAN
Author Information
- Publication Type:Journal Article
- MeSH: Antisense Elements (Genetics); pharmacology; Cell Cycle; Cell Line, Tumor; Genetic Therapy; Humans; Stomach Neoplasms; chemistry; pathology; therapy; rhoA GTP-Binding Protein; analysis; antagonists & inhibitors; physiology
- From: Chinese Journal of Oncology 2004;26(1):26-29
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the expression and possible function of RhoA in human gastric cancer cell lines.
METHODSThe expression of RhoA in human gastrointestinal cancer cell lines was detected by Western blot. Antisense plasmid of RhoA was constructed by pGEFL and transferred into gastric cancer cell line AGS by lipofectamine. Cell survival was examined by MTT assays, and cell cycle was detected by flow cytometry.
RESULTSThe expression of RhoA protein in 10 different kinds of human cancer cell lines was much higher than that in immortalized human intestinal epithelial cell line. After being transfected with antisense RhoA, with the decrease in RhoA protein expression, the growth rate of AGS was inhibited, and the number of cells in S phase was increased by 14%.
CONCLUSIONRhoA is overexpressed in many human cancer cell lines. Some of the malignant characteristics of a gastric cancer cell line can be partially reversed by inhibiting RhoA expression.
