Construction of pEgr-TNFalpha plasmid and experimental study on the effect of gene-radiotherapy on mouse melanoma.
- Author:
Cong-Mei WU
1
;
Xiu-Yi LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Combined Modality Therapy; DNA-Binding Proteins; genetics; Early Growth Response Protein 1; Female; Genetic Therapy; Immediate-Early Proteins; genetics; Interleukin-2; secretion; Killer Cells, Natural; immunology; Melanoma, Experimental; immunology; therapy; Mice; Plasmids; Transcription Factors; genetics; Tumor Necrosis Factor-alpha; genetics
- From: Chinese Journal of Oncology 2004;26(3):143-145
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe pEgr-TNFalpha plasmid was constructed to investigate the effect of gene-radiotherapy on melanoma and host immune system.
METHODSpEgr-TNFalpha plasmids were constructed and injected into tumor tissue, 36 hours later, the tumors were given 20 Gy X-ray irradiation. Tumor growth at different timepoints was record and immunologic parameters were detected 15 days later.
RESULTSFrom 3 to 15 d after pEgr-TNFalpha gene-radiotherapy the tumor growth was significantly slower than irradiation or genetherapy alone. NK activity, IL-2, TNFalpha and IL-1beta secretion activities of pEgr-TNFalpha gene-radiotherapy group and pEgr-TNFalpha gene group were higher than those of irradiation alone group significantly.
CONCLUSIONThe anti-tumor effect of pEgr-TNFalpha gene-radiotherapy is better than that of either one applied solely, and it can alleviate the lesion caused by radiation therapy.
