Enhancement of the bystander effect by tanshinone IIA in HSV-tK/GCV system is related to expression of connexin 43 mRNA.
- Author:
Guang-Qi HUANG
1
;
Yi SONG
;
Jie ZHANG
;
Yan-Rong LU
;
Lin XIAO
;
Yuan YANG
;
Yuan-Biao GUO
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents, Phytogenic; pharmacology; Bystander Effect; Cell Line, Tumor; Cell Survival; drug effects; Connexin 43; genetics; Diterpenes, Abietane; Female; Ganciclovir; pharmacology; Genetic Therapy; Humans; Phenanthrenes; pharmacology; Reverse Transcriptase Polymerase Chain Reaction; Simplexvirus; enzymology; Thymidine Kinase; genetics; Uterine Cervical Neoplasms; therapy
- From: Chinese Journal of Oncology 2004;26(3):146-149
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate enhancement of the bystander effect by tanshinone IIA (Tan) in HSV-tK/GCV system and the correlation with expression of connexin 43 mRNA.
METHODSThe cytotoxic effect in HSV-tK/GCV in cervical carcinoma cell line ME180 (ME) and ME/TK was examined by MTT assays. Cx43 mRNA expression was detected by fluor-quantitative RT-PCR.
RESULTSTan markedly increased sensitivity of ME/TK cells for GCV in HSV-tK/GCV system. In the presence of 2 micro g/ml GCV, compared with the absence of Tan (0 mol/L), an obvious decrease in survival rate was seen at any given mixture of ME and ME/TK cells exposed to 1.3 x 10(-9) mol/L Tan. Statistics showed significant difference (P < 0.05). However, enhancement of bystander mediated cell killing occurred only in the range of Tan concentrations used (1.3 x 10(-8), 1.3 x 10(-9) mol/L). RT-PCR showed that the ratio of relative copy number of Cx43 mRNA increased by 8.83 and 8.47-fold in ME cells exposed to 1.3 x 10(-8) and 1.3 x 10(-9) mol/L Tan, respectively.
CONCLUSIONFor the first time we report that in cervical carcinoma ME180 cell line, Tan possesses a remarkable enhancing role on the bystander effect in the HSV-tK/GCV system. It is associated with up-regulation of Cx43 mRNA expression.
