Combined detection of CEA mRNA and CK 19 mRNA in peripheral blood cells of patients with lung cancer.
- Author:
Xu-fen LI
1
;
Yue HU
;
Su-zhan ZHANG
;
Shu ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; blood; pathology; Adult; Aged; Carcinoembryonic Antigen; biosynthesis; blood; genetics; Carcinoma, Small Cell; blood; pathology; Carcinoma, Squamous Cell; blood; pathology; Female; Humans; Keratins; biosynthesis; blood; genetics; Lung Neoplasms; blood; pathology; Male; Middle Aged; Neoplasm Staging; RNA, Messenger; blood; genetics; Reverse Transcriptase Polymerase Chain Reaction
- From: Chinese Journal of Oncology 2004;26(6):382-384
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of CEA mRNA and CK(19) mRNA in peripheral blood cells of patients with lung cancer and evaluate its clinical significance.
METHODSPeripheral blood nucleated cells of 50 patients with lung cancer were studied by RT-PCR to detect the expression of CEA mRNA and CK(19) mRNA.
RESULTSThe combined positive rate of CEA mRNA and CK(19) mRNA in patients with lung cancer (82.0%) was significantly higher than that in patients with benign lung diseases (28.0%) and healthy volunteers (8.1%) (P < 0.001). The expression rate had no relation to the clinical staging or histological type. Compared with single detection, combined detection increased the detection rate but did not decrease the specificity.
CONCLUSIONCombined detection of CEA mRNA and CK(19) mRNA expression in peripheral blood nucleated cells increase the sensitivity of detecting hematogenous dissemination of cancer cells. Long-term survival analysis and more specimens would be helpful for evaluating its clinical significance.
