Effect of estrogen and progesterone on the expression of VEGF mRNA in epithelial ovarian cancer cell line.
- Author:
Hua LI
1
;
Hong SUN
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; metabolism; pathology; Antineoplastic Agents, Hormonal; pharmacology; Cell Line, Tumor; Dose-Response Relationship, Drug; Estradiol; pharmacology; Female; Humans; Medroxyprogesterone Acetate; pharmacology; Ovarian Neoplasms; metabolism; pathology; RNA, Messenger; biosynthesis; genetics; Vascular Endothelial Growth Factor A; biosynthesis; genetics
- From: Chinese Journal of Oncology 2004;26(5):264-267
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of 17beta-estradiol (17beta-E(2)) and medroxyprogesterone acetate (MPA) on the expression of VEGF mRNA in epithelial ovarian carcinoma cell line in vitro.
METHODSHuman epithelial ovarian carcinoma cell line SKOV3 cells were exposed to 17beta-E(2) (10(-12) approximately 10(-8) mol/L) or MPA (10(-9) approximately 10(-5) mol/L), the expression level of VEGF mRNA was determined by RT-PCR.
RESULTSThe PCR products of VEGF were mainly 606 bp and 474 bp. The expression of VEGF mRNA in the 17beta-E(2)-treated cells was significantly higher than in the untreated control (P < 0.05), the expression level of VEGF mRNA increased with the increase in 17beta-E(2) concentration. The expression of VEGF mRNA in SKOV3 cells treated with MPA (10(-9) approximately 10(-5) mol/L) was significantly lower than in the untreated control (P < 0.05), especially that of 606 bp.
CONCLUSIONEstrogen can up-regulate, while progesterone can down-regulate mRNA expression of VEGF. Further studies are needed to elucidate the mechanism.
