Inhibitory effect of tumor suppressor PTEN on cell growth of endometrial carcinoma.
- Author:
Xiao-yun WAN
1
;
Yi-fu SHI
;
Juan-qing LI
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Cell Proliferation; Endometrial Neoplasms; metabolism; pathology; Female; Gene Expression Regulation, Neoplastic; Humans; Insulin-Like Growth Factor II; pharmacology; PTEN Phosphohydrolase; Phosphoric Monoester Hydrolases; biosynthesis; genetics; physiology; Recombination, Genetic; Transfection; Tumor Suppressor Proteins; biosynthesis; genetics; physiology
- From: Chinese Journal of Oncology 2004;26(5):275-278
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the inhibitory effect of tumor suppressor PTEN on cell growth of endometrial carcinoma.
METHODSThe exogenous wild PTEN cDNA via an adenoviral vector (Ad-PTEN) was introduced into Ishikawa cells. The expression of PTEN protein was detected by Western blot. The growth of Ishikawa cells was evaluated by trypan blue exclusion method and MTT.
RESULTSThe expression of PTEN protein was induced on day 1, and greatly increasing on day 3 - 5 after Ad-PTEN infection. The expression of PTEN significantly inhibited the growth of Ishikawa cells, and also significantly inhibited the growth of Ishikawa cells induced by IGF-II.
CONCLUSIONAdenovirus-mediated introduction of exogenous PTEN into human endometrial carcinoma cells can induce growth suppression. PTEN gene may be a novel therapeutic agent for endometrial carcinoma.
