Glutathione peroxidase activity in cell cultures from different regions of human epididymis.
- Author:
Enrique CASTELLÓN
1
;
Hernán RIOSECO
;
Juan ROJAS
;
Michel ROYER
;
Eduardo SALAS
;
Héctor CONTRERAS
;
Christian HUIDOBRO
Author Information
- Publication Type:Journal Article
- MeSH: Aged; Androgen Antagonists; pharmacology; Cell Culture Techniques; Epididymis; enzymology; Flutamide; pharmacology; Glutathione Peroxidase; drug effects; metabolism; Humans; Male; Middle Aged; Testosterone; metabolism
- From: Asian Journal of Andrology 2005;7(1):33-37
- CountryChina
- Language:English
-
Abstract:
AIMTo study the secretory activity and androgen regulation of glutathione peroxidase (GPx) in epithelial cell cultures from human epididymis.
METHODSTissue was obtained from patients undergoing therapeutic orchidectomy for prostatic cancer. Epithelial cell cultures were obtained from the caput, corpus and cauda epididymides. Enzymatic activity was measured in conditioned media by colorimetric methods in absence or presence of 1, 10 or 100 nmol/L testosterone. The effect of 1 micromol/L flutamide was also evaluated.
RESULTSGPx activity was higher in cultures from corpus and cauda than caput epididymidis. The presence of different concentrations of testosterone increase enzyme activity in cell cultures from all epididymal regions. Addition of flutamide reverses the androgen dependent increase of GPx activity.
CONCLUSIONGPx activity is secreted from human epididymal cells in a region dependent manner and is regulated by androgens.
