Expression of a novel dipeptidyl peptidase 8 (DPP8) transcript variant, DPP8-v3, in human testis.
- Author:
Hui ZHU
1
;
Zuo-Min ZHOU
;
Li LU
;
Min XU
;
Hui WANG
;
Jian-Min LI
;
Jia-Hao SHA
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Amino Acid Sequence; Base Sequence; Blotting, Southern; DNA Primers; DNA, Complementary; Dipeptidases; chemistry; genetics; Gene Expression Profiling; Humans; Male; Molecular Sequence Data; Nucleic Acid Hybridization; RNA, Messenger; genetics; Reverse Transcriptase Polymerase Chain Reaction; Sequence Homology, Amino Acid; Testis; embryology; enzymology; growth & development
- From: Asian Journal of Andrology 2005;7(3):245-255
- CountryChina
- Language:English
-
Abstract:
AIMTo investigate the role of a novel dipeptidyl peptidase 8 transcript variant (DPP8-v3) gene in testis development and/or spermatogenesis.
METHODSA human testis cDNA microarray was hybridized with mRNA of human adult and fetal testes. Differentially expressed clones were sequenced and characterized and their expression was analyzed by real-time reverse transcription polymerase chain reaction (RT-PCR) and Southern-blot analysis.
RESULTSA new transcript variant of the human dipeptidyl peptidase (DPP8), exhibiting a 5-fold higher expression level in human adult than that in fetal testes, was cloned and was named DPP8 variant 3 (DPP8-v3). The full-length sequence of DPP8-v3 was 3,030 bp, encoding a protein of 898 amino acids.
CONCLUSIONDPP8-v3 is a novel human DPP8 transcript variant highly expressed in the adult testis. Similar to DPPIV, DPP8-v3 may play a key role in the immunoregulation of testes and accordingly may influence spermatogenesis and male fertility.
