Effects on the quality of frozen-thawed alpaca (Lama pacos) semen using two different cryoprotectants and extenders.

Alexei SANTIANI; Wilfredo HUANCA; Rómúlo SAPANA; Teodosio HUANCA; Néstor SEPÚLVEDA; Raúl SÁNCHEZ

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Effects on the quality of frozen-thawed alpaca (Lama pacos) semen using two different cryoprotectants and extenders.

Author: Alexei SANTIANI ¹ ; Wilfredo HUANCA ; Rómúlo SAPANA ; Teodosio HUANCA ; Néstor SEPÚLVEDA ; Raúl SÁNCHEZ
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1. Veterinary Institute for Tropical and Highland Research, Faculty of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, P.O. Box 03-5034, Lima 41, Perú.
Publication Type:Journal Article
MeSH: Animals; Camelids, New World; Cryoprotective Agents; administration & dosage; Freezing; Male; Semen; Semen Preservation; Sperm Motility
From: Asian Journal of Andrology 2005;7(3):303-309
CountryChina
Language:English
Abstract:
AIMTo evaluate two extenders and two cryoprotectant agents (CPA) for alpaca semen cryopreservation.
METHODSSemen samples were obtained from four adult alpacas (Lama pacos) and frozen using extender I (TRIS, citrate, egg yolk and glucose) or extender II (skim milk, egg yolk and fructose), each containing either glycerol (G) or ethylene glycol (EG) as CPA. Consequently, four groups were formed: 1) extender I-G; 2) extender I-EG; 3) extender II-G; and 4) extender II-EG. Semen was diluted in a two-step process: for cooling to 5 degrees (extenders without CPA), and for freezing (extenders with CPA). Viability and acrosome integrity were assessed using trypan blue and Giemsa stains.
RESULTSWhen compared, the motility after thawing was higher (P >0.05) in groups II-EG (20.0% +/- 6.7%) and II-G (15.3 % +/- 4.1% ) than that in groups I-G (4.0 % +/- 1.1%) and I-EG (1.0 % +/- 1.4%). Viable spermatozoa with intact acrosomes in groups II-EG (18.7 % +/- 2.9%) and II-G (12.7 % +/- 5.9%) were higher than that in groups I-G (5.7% +/- 1.5%) and I-EG (4.0% +/- 1.0%).
CONCLUSIONThe skim milk- and egg yolk-based extenders containing ethylene glycol or glycerol to freeze alpaca semen seems to promote the survival of more sperm cells with intact acrosomes than the other extenders.