Effect of NECL1 on the proliferation of T98G glioma cell line.
- Author:
Jing GAO
1
;
Tao CHEN
;
Bin YIN
;
Yan-Hua GONG
;
Bo-Qin QIANG
;
Jian-Gang YUAN
;
Xiao-Zhong PENG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; genetics; physiology; Blotting, Western; Cell Line, Tumor; Cell Proliferation; Glioma; metabolism; pathology; Humans; Immunoglobulins; genetics; metabolism; In Vitro Techniques; Membrane Proteins; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Transfection
- From: Acta Academiae Medicinae Sinicae 2008;30(3):280-283
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the regulation role of tumor suppressor NECL1 on the proliferation of glioma cell line.
METHODSWe detected the expression level of NECL1 in human normal brain tissue and glioma cell lines using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot. T98G cell line in which NECL1 was silent and whose transfection efficiency was relatively high as target cell was chosen, and the effect of NECL1 on the proliferation of T98G cell line in vitro was detected by using cell growth curve, flow cytometry, and Hoechst staining.
RESULTSNECL1 was abrogated or markedly reduced in 6 glioma cell lines. When NECL1 was overexpressed in T98G cell line, the cell growth rate obviously decreased and the number of apoptotic cells remarkably increased when compared with the control group.
CONCLUSIONNECL1 may inhibit the proliferation of T98G cells by inducing its apoptosis.
