Autophagy in fibrotic and postoperative remnant liver in rat.
- Author:
Yi-lei MAO
1
;
Rong-rong CHEN
;
Hua-yu YANG
;
Jing-chun ZHANG
;
Yi-dan ZHANG
;
Jian-hui MA
;
Xin-ting SANG
;
Xin LU
;
Shou-xian ZHONG
;
Jie-fu HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Alanine Transaminase; blood; Animals; Aspartate Aminotransferases; blood; Autophagy; Disease Models, Animal; Hepatectomy; Humans; Liver; metabolism; pathology; physiopathology; surgery; Liver Cirrhosis; metabolism; pathology; physiopathology; surgery; Male; Proliferating Cell Nuclear Antigen; metabolism; Random Allocation; Rats; Rats, Wistar
- From: Acta Academiae Medicinae Sinicae 2008;30(4):421-425
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the characteristics of autophagy in fibrotic and postoperative remnant liver.
METHODSMale Wistar rats were randomly divided into three groups: control group; fibrosis group, which received the solution of CCl4 in oil twice a week for 5 weeks; and hepatectomy group, which underwent 70% hepatectomy. Liver tissues and plasma were harvested 18 hours after the surgery. The rats' general conditions and plasma liver function were observed. Histopathological characteristics and regeneration were observed with microscope and transmission electron microscope. Qualitative analysis of autophagosome was made base on the data from transmission electron microscope.
RESULTSCompared with the control group, plasma total protein and albumin level significantly decreased in the fibrosis group (P < 0.01). Proliferating cell nuclear antigen (PCNA) index was 85%-95% in the fibrosis group. Plasma alanine aminotransferase and aspartate aminotransferase levels significantly increased in the hepatectomy group compared with the control group (P < 0.01), while the autophagical index significantly decreased in both the fibrosis group and hepatectomy group compared with the control group (-95%, P < 0.01; -19%, P < 0.05, respectively). PCNA index was 20%-30% in the hepatectomy group.
CONCLUSIONSAutophagy is weakened after fibrosis and hepatectomy, although it differs between these two processes. Proper regulation of autophagy may help facilitate the recovery of the residual liver function after hepatectomy.
