MicroRNA differential expression profile during differentiation of embryonic stem cells towards hepatocytes induced by sodium butyrate.
- Author:
Ya-jin CHEN
1
;
Jun MIN
;
Chang-zhen SHANG
;
Meng REN
;
Xiao-xiong PENG
;
Jun CAO
;
Ji-sheng CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Butyrates; pharmacology; Cell Differentiation; drug effects; Cells, Cultured; Embryonic Stem Cells; cytology; drug effects; metabolism; Gene Expression; drug effects; Hepatocytes; cytology; drug effects; metabolism; Mice; MicroRNAs; genetics; metabolism
- From: Acta Academiae Medicinae Sinicae 2008;30(4):469-473
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the expression profile of microRNAs during the course of embryonic stem cells differentiation towards hepatocytes induced by sodium butyrate.
METHODSTotal RNA was extracted from embryonic stem cells on day 0, 6, and 9 during cell differentiation, and microRNA was isolated from the total RNA. Microarray analysis of microRNA expression was performed to detect the different expression levels of microRNA among the indicated time points (day 0, 6, and 9).
RESULTSCompared with the microRNA expression level on day 0 of cell differentiation, 17 different microRNAs exhibited higher expressions both on day 6 and day 9. Twenty-two and 27 microRNA demonstrated lower expressions on day 6 and day 9, respectively. Further analysis revealed that 15 microRNA among the above microRNAs with significant differential expression may keep close interation with histone deacetylase.
CONCLUSIONDuring the course of embryonic stem cells differentiation towards hepatocytes induced by sodium butyrate, histone deacetylase and its relevant microRNAs may play important roles in cell differentiation.
