Detection of deletion of the long arm of chromosome 13 and translocation of immunoglobulin heavy chain gene by interphase fluorescence in situ hybridization in patients with multiple myeloma.
- Author:
Wan-ling SUN
1
;
Yong-ji WU
;
Hui LI
;
Xuan WANG
;
Jun-ling ZHUANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aged, 80 and over; Chromosome Deletion; Chromosomes, Human, Pair 13; genetics; Chromosomes, Human, Pair 14; genetics; Female; Humans; Immunoglobulin Heavy Chains; genetics; In Situ Hybridization, Fluorescence; Interphase; Male; Middle Aged; Multiple Myeloma; genetics; Translocation, Genetic
- From: Acta Academiae Medicinae Sinicae 2008;30(4):485-490
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the clinical significance of the deletion of the long arm of chromosome 13 [del (13 q) ] and translocation of immunoglobulin heavy chain gene [t (14 q) I in multiple myeloma (MM) patients.
METHODSMyeloma cells were isolated from hone marrow by direct immunomagnetic cell sorting and interphase fluorescence in situ hybridization (FISH) was performed in 24 MM patients to detect del (l3q) and t (l4q).
RESULTSThe positive rates of del (l3q) and t (l4q) were 45.83% and 37.50% respectively. Five patients (20.83%) had both two abnormalities and 15 patients (62.50%) had at least one abnormality. Univariate analysis showed that the positive rates of del (l3q) were 35.71% and 66.67% in responders and non-responders (P = 0.214) and the positive rates of t (l4q) were 21.43% and 66. 67% in responders and non-responders (P = 0.077). Multivariate analysis showed that del (13q) (OR = 5.761, 95% CI 0.500-66.391, P = 0.160), t (14q) (OR = 6.576, 95% CI 0.580-74.614, P = 0.129), and corrected serum calcium level (OR = 8.080, 95% CI 0.738-88.427, P = 0.087) were relatively independent negative factors for response to therapy, with the corrected serum calcium level being the strongest reversely-correlated factor.
CONCLUSIONSInterphase FISH is a sensitive method to investigate the cytogenetics of MM. Del (13q), t (14q), and corrected serum calcium level can be used to predict treatment response and prognosis.
