Molecular characterization of vancomycin-resistant Enterococci.
- Author:
He WANG
1
;
Ying-Chun XU
;
Xiu-Li XIE
;
Peng WANG
;
Ren-Yuan ZHU
;
Xiao-Jiang ZHANG
;
Hui WANG
;
Min-Jun CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Bacterial Proteins; genetics; Bacterial Typing Techniques; Drug Resistance, Multiple, Bacterial; Enterococcus faecium; classification; drug effects; genetics; isolation & purification; Gram-Positive Bacterial Infections; microbiology; Humans; Vancomycin Resistance
- From: Acta Academiae Medicinae Sinicae 2008;30(5):521-524
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the homology and resistant mechanism of vancomycin-resistant Enterococci (VRE) isolates.
METHODSA total of 9 VRE isolates were collected from 2006 to 2007 at PUMC hospital. The susceptibility of these isolates to 10 different antibiotics including vancomycin was tested by E-test. These strains were processed by brain heart infusion agar screening in the presence of vancomycin (6 microg/ml), and were analyzed for genotypic characteristics using the multiplex PCR. The homology of the isolates was determined by pulsed-field gel electrophoresis (PFGE).
RESULTSAll the 9 VRE isolates were identified as Enterococci faecium. The visual analysis of PFGE patterns revealed 6 different PFGE types. The vanA gene was confirmed by PCR and sequencing in 9 VRE isolates, which were consistent between phenotype and genotype for glycopeptides resistance.
CONCLUSIONSOnly vanA genotype was detected in PUMC hospital. Clonal dissemination, horizontal gene transfer, and the selective pressure of antimicrobial agents may contribute to the increase of VRE.
