Expression pattern of polycomb gene Nspc1 at the early developmental stage in zebrafish.
- Author:
Xu-Dong WU
1
;
Min ZHANG
;
Yan-Hua GONG
;
Bo-Qin QIANG
;
Jian-Gang YUAN
;
An-Ming MENG
;
Xiao-Zhong PENG
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Animals; Gene Expression Regulation, Developmental; Humans; Mice; Molecular Sequence Data; Nerve Tissue; growth & development; metabolism; Polycomb Repressive Complex 1; Repressor Proteins; chemistry; genetics; metabolism; Sequence Alignment; Zebrafish; genetics; growth & development; metabolism; Zebrafish Proteins; chemistry; genetics; metabolism
- From: Acta Academiae Medicinae Sinicae 2008;30(5):550-553
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the expression pattern of Polycomb gene Nspc1 at the early developmental stage in zebrafish.
METHODSIn situ hybridization probe for Nspc1 was designed according to the GenBank information. Collecting zebrafish embryos at different stages including one cell stage, two-cell stage, bud stage, and somites stage, we hybridized them with the prepared probe. Then the hybridization signals at different intervals were observed and photographed at the right time.
RESULTSNspc1 was expressed globally at the early stage. Its expression specificity began at the somites stage, mainly in the nervous system of the head.
CONCLUSIONNspc1 may play essential roles in the early stage development of zebrafish, especially in the nervous system.
