Three-step purification of preparative-scale antiCD20 (Fab')2.
- Author:
Jin-Hong WANG
1
;
Ming YANG
;
Dong-Mei FAN
;
Yuan-Sheng XU
;
Dong-Sheng XIONG
;
Chun-Zheng YANG
Author Information
- Publication Type:Journal Article
- MeSH: Antibodies; immunology; isolation & purification; Antigens, CD20; immunology; Chromatography; methods; Humans; Immunoglobulin Fab Fragments; immunology; isolation & purification
- From: Acta Academiae Medicinae Sinicae 2008;30(5):622-625
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a three-step purification method of preparative-scale antiCD20 (Fab')2 using AKTA prime.
METHODSAntiCD20 (Fab')2 was extracted by hyperosmotic solution and then purified by CM sepharose FF, phenyl sepharose FF, and protein G sepharose FF.
RESULTSAround 8 mg anti-CD20 (Fab')2, whose purification was 96.678%, was purified. The antigen-binding activity of antiCD20 (Fab')2 was similar to that of antiCD20 (Fab')2 purified by protein G sepharose FF and S-100.
CONCLUSIONThe three-step purification method can obtain high-purity preparative-scale antiCD20 (Fab')2 in a simple way.
