Di-(n-butyl)-phthalate-induced oxidative stress and depression-like behavior in mice with or without ovalbumin immunization.

Hao Xiao ZUO; Jin Quan LI; Bing HAN; Chen Juan KE; Xu Dong LIU; Yu Chao ZHANG; Li LI; Xu YANG

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Di-(n-butyl)-phthalate-induced oxidative stress and depression-like behavior in mice with or without ovalbumin immunization.

Author: Hao Xiao ZUO ¹ ; Jin Quan LI ¹ ; Bing HAN ² ; Chen Juan KE ¹ ; Xu Dong LIU ¹ ; Yu Chao ZHANG ¹ ; Li LI ¹ ; Xu YANG ¹
Author Information

1. Section of Environmental Biomedicine, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, Hubei, China.
2. Department of Molecular Pharmacology, University Centre for Pharmacy, University of Groningen, Groningen 9713AV, the Netherlands.
Publication Type:Journal Article
Keywords: Atopic allergy; Behavioral tests; Depression; Di (n-butyl) phthalate; Mice; Oxidative stress
MeSH: Animals; Behavior, Animal; drug effects; Body Weight; Depression; blood; chemically induced; immunology; Dibutyl Phthalate; immunology; toxicity; Environmental Pollutants; immunology; toxicity; Hydrocortisone; Hypersensitivity, Immediate; blood; complications; Immunization; Immunoglobulin E; blood; Interleukin-4; blood; Male; Mice; Mice, Inbred BALB C; Ovalbumin; Oxidative Stress
From: Biomedical and Environmental Sciences 2014;27(4):268-280
CountryChina
Language:English
Abstract:
OBJECTIVETo investigate the relationship between atopic allergy and depression and the role of DBP in the development of depression.
METHODSBALB/c mice were randomly divided into eight groups: saline; ovalbumin (OVA)-immunized; saline+DBP (0.45 mg/kg•d); saline+DBP (45 mg/kg•d); DBP (0.45 mg/kg•d) OVA-immunized; DBP (45 mg/kg•d) OVA-immunized; saline+hydrocortisone (30 mg/kg•d); and hydrocortisone (30 mg/kg•d)-exposed OVA-immunized. Behavior (e.g. open-field, tail suspension, and forced swimming tests), viscera coefficients (brain and spleen), oxidative damage [e.g. reactive oxygen species (ROS), malondialdehyde (MDA), and glutathione (GSH)], as well as levels of IgE and IL-4, were then analyzed.
RESULTSIn the saline and OVA groups, the degree of depression symptoms in mice increased with increasing DBP concentration. Additionally, the OVA-immunity groups were associated with more serious depressive behavior compared with the same exposure concentration in the saline group. Oxidative damage was associated with a dose-dependent increase in DBP in the different groups. IL-4 and IgE levels were associated with low-dose DBP stimulation, which changed to high-dose inhibition with increasing DBP exposure, possibly due to spleen injury seen at high DBP concentrations.
CONCLUSIONDevelopment of an atopic allergy has the potential to increase the risk of depression in mice, and it seems that DBP helps OVA to exert its effect in our present model. Moreover, the results of our study implicate a certain connection between brain oxidative stress and depression, which deserves a further exploration.