Effect of sterilization methods on cell binding activity of surface-immobilized fibronectin.

Author: Hai Bo XING ¹ ; Qin PAN ² ; Kai Feng WANG ² ; Fang LOU ² ; Da LI ²
Author Information

1. Intensive Care Unit, Xiasha Hospital, Hangzhou 310019, Zhejiang, China.
2. Department of Medical Oncology, Sir Run Run Shaw Hospital Affiliated to Zhejiang University Medical School, Hangzhou 310019, Zhejiang, China.
Publication Type:Letter
MeSH: Cell Adhesion; radiation effects; Fibronectins; radiation effects; Filtration; Prostheses and Implants; microbiology; Sterilization; methods; Ultraviolet Rays
From: Biomedical and Environmental Sciences 2014;27(5):391-395
CountryChina
Language:English
Abstract: The effect of sterilization methods on biological activity of fibronectin on the surface of biomaterials was elaborated in the present study. Sterile protein- modified biomaterials were fabricated by microfilter filtration and UV irradiation, respectively. UV irradiation altered the conformation of surface- adsorbed fibronectin and further affected the attachment, morphology and biological function of endothelial cells. However, microfilter filtration did not to change the normal conformation of fibronectin, or the proliferation and biological function of endothelial cells, indicating that microfilter filtration sterilization is the most suitable method for protein-substrate.